• Catalog Number:

  E1A7211A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human c-Rel around the phosphorylation site of Serine 503.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q04864

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Specificity Sensitivity:

  c-Rel (Phospho-Ser503) Antibody detects endogenous levels of c-Rel only when phosphorylated at Serine 503.

• References:

  Martin A G, et al. (2000) J Biol Chem. 275(32): 24383-24391.

Immunohistochemical analysis of paraffin-embedded breast carcinoma, Left: Using c-Rel (Phospho- Ser503) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.

Western blot analysis of extracts from MDA-MB-435 cells. Line1: Using c-Rel(Ab-503) Antibody; Line2: The same antibody preincubated with synthesized peptide.