• Catalog Number:

  E1A1031A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GRB10 around the phosphorylation site of tyrosine 67 (S-L-YP-S-A).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q13322

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  GRB10 adaptor protein, GRBIR, Growth factor receptor-bound protein 10, Insulin receptor binding protein GRB-IR, KIAA0207

• Specificity Sensitivity:

  GRB10 (Phospho-Tyr67) antibody detects endogenous levels of GRB10 only when phosphorylated at tyrosine 67.

• References:

  Liu F., Proc. Natl. Acad. Sci. U.S.A. 92:10287-10291(1995). Angrist M., Oncogene 17:3065-3070(1998). Nagase T., DNA Res. 3:321-329(1996).

• All Sites:

  Human: Tyr67

P-peptide - + Western blot analysis of extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins), using GRB10 (Phospho-Tyr67) antibody. Immunofluorescence analysis of HepG2 cells, using GRB10 (Phospho-Tyr67) antibody.