• Catalog Number:

  E1A1021A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AurB around the phosphorylation site of threonine 232 (R-K-TP-M-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q96GD4

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  AIK2, AIM-1, AIM1, ARK2, AURKB, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, EC 2.7.11.1, STK-1, STK12, Serine/threonine protein kinase 12

• Specificity Sensitivity:

  AurB (Phospho-Thr232) antibody detects endogenous levels of AurB only when phosphorylated at threonine 232.

• References:

  Shindo M., Biochem. Biophys. Res. Commun. 244:285-292(1998). Tatsuka M., Cancer Res. 58:4811-4816(1998). Kimura M., Cytogenet. Cell Genet. 82:147-152(1998).

• All Sites:

  Human: Thr232; Mouse: Thr237; Rat: Thr235

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using AurB (Phospho-Thr232) antibody. Western blot analysis of extracts from COS7 cells, treated with Nocodazole (1ug/ml, 16hours), using AurB (Phospho-Thr232) antibody.

P-peptide - + Immunofluorescence analysis of HepG2 cells, using AurB (Phospho-Thr232) antibody.