• Catalog Number:

  E1A1030A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GIRK1/KIR3.1/KCNJ3 around the phosphorylation site of serine 185 (K-M-SP-Q-P).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P48549

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  G protein-activated inward rectifier potassium channel 1, IRK3, Inward rectifier K channel Kir3.1, KCNJ3, Kir3.1, Potassium channel, inwardly rectifying, subfamily J, member 3

• Specificity Sensitivity:

  GIRK1/KIR3.1/KCNJ3 (Phospho-Ser185) antibody detects endogenous levels of GIRK1/KIR3.1/KCNJ3 only when phosphorylated at serine 185.

• References:

  Chan K.W., J. Gen. Physiol. 107:381-397(1996). Schoots O., Brain Res. Mol. Brain Res. 39:23-30(1996). The MGC Project Team; Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Ser185; Mouse: Ser185; Rat: Ser185

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using GIRK1/KIR3.1/KCNJ3 (Phospho-Ser185) antibody. Western blot analysis of extracts from RAW264.7 cells, treated with Insulin (0.01U/ml, 15mins), using GIRK1/KIR3.1/KCNJ3 (Phospho-Ser185) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using GIRK1/KIR3.1/KCNJ3 (Phospho-Ser185) antibody.