• Catalog Number:

  E1A1017A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Vinculin around the phosphorylation site of tyrosine 821 (S-G-YP-R-I).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q64727

• Applications:

  IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  Metavinculin, VCL, VINC

• Specificity Sensitivity:

  Vinculin (Phospho-Tyr821) antibody detects endogenous levels of Vinculin only when phosphorylated at tyrosine 821.

• References:

  Tricia A. Holwell, J. Lipid Res., Aug 1999; 40: 1440. M Galou, J. Cell Biol., May 1996; 133: 853. PV Hornbeck, J. Immunol., Oct 1993; 151: 4013. M Ishizaki, Invest. Ophthalmol. Vis. Sci., Nov 1993; 34: 3320.

• All Sites:

  Human: Tyr821; Mouse: Tyr821; Rat: Tyr822

P-peptide - +

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Vinculin (Phospho-Tyr821) antibody. Immunofluorescence analysis of HepG2 cells, using Vinculin (Phospho-Tyr821) antibody.