• Catalog Number:

  E1A1084A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Kir6.2 around the phosphorylation site of threonine 224 (K-T-TP-S-P).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q14654

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  ATP-sensitive inward rectifier potassium channel 11, IKATP, IRK11, Inward rectifier K channel Kir6.2, KCNJ11, Potassium channel, inwardly rectifying, subfamily J, member 11

• Specificity Sensitivity:

  Kir6.2 (Phospho-Thr224) antibody detects endogenous levels of Kir6.2 only when phosphorylated at threonine 224.

• References:

  Laurianne van Bever, Am J Physiol Heart Circ Physiol, Aug 2004; 287: H850 - H859. Hong-Shuo Sun, J Neurophysiol, Apr 2006; 95: 2590 - 2601. Peter Proks, J. Physiol., Jan 1999; 514: 19 - 25.

• All Sites:

  Human: Thr224; Mouse: Thr224

P-peptide - + Western blot analysis of extracts from HeLa cells, using Kir6.2 (Phospho-Thr224) antibody. Immunofluorescence analysis of HuvEc cells, using Kir6.2 (Phospho-Thr224) antibody.