• Catalog Number:

  E011138-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human cdc25A around the phosphorylation site of serine 75 (M-G-SP-S-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P30304

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  cdc25A (phospho-Ser75) antibody detects endogenous levels of cdc25A only when phosphorylated at serine 75.

• References:

  Jin J, et al. (2003) Genes 17(24): 3062-74. Shimuta K, et al. (2002) EMBO J 21(14): 3694-703.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using cdc25A (phospho-Ser75) antibody (E011138).

Western blot analysis of extracts from A2780 cells using cdc25A (Ab-75) antibody (E021163, Lane 1 and 2) and cdc25A (phospho-Ser75) antibody (E011138, Lane 3 and 4)