• Catalog Number:

  E1A0023A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKCζ around the phosphorylation site of threonine 410 (T-S-TP-F-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q05513

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 IF: 1:100~1:500 ELISA: 1:20000

• Alter Name:

  EC 2.7.11.13, KPCZ, PKC-zeta, PKC2, PRKCZ, Protein kinase C zeta type, kinase PKC-zeta, nPKC-zeta

• Specificity Sensitivity:

  PKCζ (phospho-Thr410) antibody detects endogenous levels of PKCζ only when phosphorylated at threonine 410.

• References:

  Todd E. Fox, J. Biol. Chem., Feb 2007; 10.1074/jbc.M700082200. Katherine A. Fitzgerald, J. Immunol., Feb 2000; 164: 2053. Koichi Asanuma, J. Immunol., Mar 2004; 172: 3922. Ashish Bhattacharjee, J. Immunol., Sep 2006; 177: 3771 - 3781.

• All Sites:

  Human: Thr410; Mouse: Thr410; Rat: Thr410

P-peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCζ (phospho-Thr410) antibody.

Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 30mins), using PKCζ (phospho-Thr410) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using PKCζ (phospho-Thr410) antibody.