• Catalog Number:

  E1A0019A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MEF2A around the phosphorylation site of serine 408 (P-I-SP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q02078

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 IF: 1:100~1:500 ELISA: 1:40000

• Alter Name:

  MEF2, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1

• Specificity Sensitivity:

  MEF2A (phospho-Ser408) antibody detects endogenous levels of MEF2A only when phosphorylated at serine 408.

• References:

  Cyril Rauch, Am J Physiol Cell Physiol, Mar 2005; 288: C593 - C605. OI Ornatsky, Nucleic Acids Res., Jul 1999; 27: 2646 - 2654. David P. Sparling, Am J Physiol Endocrinol Metab, Apr 2007; 292: E1149 - E1156. Eric N. Olson Sci, Aging Knowl. Environ., Dec 2003; 2003: 33.

• All Sites:

  Human: Ser408; Mouse: Ser406; Rat: Ser400

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEF2A (phospho-Ser408) antibody.

Western blot analysis of extracts from Hela cells treated with PMA (125ng/ml, 30mins), using MEF2A (phospho-Ser408) antibody.

PMA + - Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using MEF2A (phospho-Ser408) antibody (#11319).