Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Cortactin around the phosphorylation site of tyrosine 466 (P-V-Y-E-T).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Cortactin (Ab-466) antibody detects endogenous levels of total Cortactin protein.
References:
Julie A. Head, Mol. Biol. Cell, Aug 2003; 14: 3216.
Cherry Luo, J. Biol. Chem., Oct 2006; 281: 30081 - 30093.
Jianwei Zhu, J. Biol. Chem., Apr 2007; 10.1074.
David Hattan, J. Biol. Chem., Oct 2002; 277: 38596 - 38606.
P-peptide - +
Immunohistochemical analysis of paraffin-embedded human breast cacinoma tissue using Cortactin (Ab-466) antibody (#B7050).
Western blot analysis of extracts from cos-7 cells, using Cortactin (Ab-466) antibody (#B7050, Line 1 and 2).