• Catalog Number:

  E1A0008A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of threonine 678 (K-R-TP-L-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P00533

• Applications:

  WB: 1:300~1:1000 IF: 1:100~1:500 ELISA: 1:40000

• Alter Name:

  EC 2.7.10.1, ERBB1, Epidermal growth factor receptor precursor, Receptor protein-tyrosine kinase ErbB-1, kinase EGFR

• Specificity Sensitivity:

  EGFR (phospho-Thr678) antibody detects endogenous levels of EGFR only when phosphorylated at threonine 678.

• References:

  Roland Reinehr, FASEB J, Feb 2003; 10: 1096. Natsumi Yamamoto, J. Cell Sci., Nov 2006; 119: 4623 - 4633. James G. Christensen Clin, Cancer Res., Dec 2001; 7: 4230. Hideki Katayama, AACR Meeting Abstracts, Mar 2004; 2004: 1074. Ali Tadlaoui Hbibi, AACR Meeting Abstracts, Apr 2006; 2006: 1177 Komal Raina, AACR Meeting Abstracts, Apr 2006; 2006: 752 - 753.

• All Sites:

  Human: Thr678; Mouse: Thr680

P-peptide - + Western blot analysis of extracts from cos7 cells treated with EGF (200ng/ml, 30mins), using EGFR (phospho-Thr678) antibody. Immunofluorescence analysis of HuvEc cells, using EGFR (phospho-Thr678) antibody.