• Catalog Number:

  E1A7113A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ICAM-1 around the phosphorylation site of tyrosine 512 (K-K-YP-R-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P05362

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Alter Name:

  CD54 antigen, ICA1, ICAM1, Intercellular adhesion molecule-1 precursor, Major group rhinovirus receptor

• Specificity Sensitivity:

  ICAM-1 (phospho-Tyr512) antibody detects endogenous levels of ICAM-1 only when phosphorylated at throsine 512.

• References:

  C Chirathaworn, J. Immunol., Dec 1995; 155: 5479. Ruth Lyck, Blood, Nov 2003; 102: 3675 - 3683. Robert Blaber, J. Am. Soc. Nephrol., Jan 2003; 14: 116. Sandrine Etienne-Manneville, J. Immunol., Sep 2000; 165: 3375.

• All Sites:

  Human: Tyr512

Western blot analysis of extracts from Hela cells treated with TNF-α (20ng/ml, 5 mins), using ICAM-1 (phospho-Tyr512) antibody, Line 1 and 2).