• Catalog Number:

  E1A0006A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CREB around the phosphorylation site of serine 142 (D-L-SP-S-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P16220

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 IF: 1:100~1:500 ELISA: 1:40000

• Alter Name:

  CREB-1, CREB1, cAMP-response element binding protein

• Specificity Sensitivity:

  CREB (phospho-Ser142) antibody detects endogenous levels of CREB only when phosphorylated at serine 142.

• References:

  Anjali Rajadhyaksha, J. Neurosci., Aug 1999; 19: 6348. Brian F. Clem, Endocrinology, Mar 2005; 146: 1348 - 1356. Boyoung Lee, J. Neurosci., Feb 2005; 25: 1137 - 1148. Lance Zirpel, J. Neurosci., Aug 2000; 20: 6267.

• All Sites:

  Human: Ser142; Mouse: Ser142; Rat: Ser142

P-peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using CREB (phospho-Ser142) antibody.

Western blot analysis of extracts from HeLa cells treated with PMA (125ng/ml, 30mins), using CREB (phospho-Ser142) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using CREB (phospho-Ser142) antibody.