Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Arachidonate 5 lipoxygenase around the phosphorylation site of serine 271 (Q-L-SP-L-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Arachidonate 5 Lipoxygenase (Ab-271) antibody detects endogenous levels of total Arachidonate 5 lipoxygenase protein.
References:
RJ Boado, PNAS, Oct 1992; 89: 9044.
K Ochi, J. Biol. Chem., May 1983; 258: 5754 - 5758.
N Ueda, J. Biol. Chem., Jun 1986; 261: 7982 - 7988.
Jagadananda Ghosh, PNAS, Oct 1998; 95: 13182.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Arachidonate 5 Lipoxygenase (Ab-271) antibody.
Western blot analysis of extracts from HuvEc cells, using Arachidonate 5 Lipoxygenase (Ab-271) antibody.