• Catalog Number:

  E1A10316C-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from internal of human HMGB1.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P09429

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  HMG-1, HMG1, High mobility group protein 1, SBP-1, amphoterin, high-mobility group box 1, sulfoglucuronyl carbohydrate binding protein high-mobility group (nonhistone chromosomal) protein 1

• Specificity Sensitivity:

  HMGB1 antibody detects endogenous levels of total HMGB1 protein.

• References:

  Wen L., Nucleic Acids Res. 17:1197-1214(1989). Ferrari S., Genomics 35:367-371(1996). Rasmussen R.K., Electrophoresis 18:588-598(1997). ------------------------------------------------------------------------------------------------------------------------------------------

Western blot analysis of extracts from Jurkat cells, HepG2 cells, COLO205 cells and 293 cells, using HMGB1 antibody.