Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human p300 around the phosphorylation site of serine 89 (S-G-SP-S-P).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
Q09472
Applications:
WB: 1:500~1:1000 ELISA: 1:1000
Other Names:
E1A-associated protein p300, EC 2.3.1.48, EP300, P300 transcription coactivator homolog
Specificity Sensitivity:
p300 (Ab-89) antibody detects endogenous levels of total p300 protein.
References:
P Yaciuk, Mol. Cell. Biol., Apr 1991; 11: 2116 - 2124.
JM Routes, J. Immunol., Feb 1996; 156: 1055.
Michelle L. Kingsley-Kallesen, J. Biol. Chem., Nov 1999; 274: 34020.
Leandro Cerchietti, Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 1413.
Western blot analysis of extracts from 293 cells, using p300 (Ab-89) antibody.