Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human Caspase 4/5.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P49662/P51878
Applications:
WB: 1:500~1:1000 ELISA: 1:40000
Other Names:
DNA primase small subunit; DNA primase 49 kDa subunit; p49
Specificity Sensitivity:
Caspase 4/5 (p20, Cleaved-Asp270/Asp311) antibody detects endogenous levels of fragment of activated Caspase 4/5 resulting from cleavage adjacent to Asp270/Asp311.
References:
Faucheu C., EMBO J. 14:1914-1922(1995).
Munday N.A., J. Biol. Chem. 270:15870-15876(1995).
Kamens J., J. Biol. Chem. 270:15250-15256(1995).
Western blot analysis of extracts from Jurkat cells, using Caspase 4/5 (p20, Cleaved-Asp270/Asp311) antibody.