• Catalog Number:

  E1A7255A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Zap-70 around the phosphorylation site of tyrosine 319 (S-P-YP-S-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P43403

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Alter Name:

  70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70, ZAP-70, ZAP70, kinase ZAP70

• Specificity Sensitivity:

  Zap-70 (Phospho-Tyr319) antibody detects endogenous levels of Zap-70 only when phosphorylated at tyrosine 319. This antibody may cross-react with Syk when phosphorylated at equivalent sites.

• References:

  Salomon AR, et al. (2003) Proc Natl Acad Sci USA; 100(2): 443-448. Ku GM, et al. (2001) EMBO J; 20(3): 457-465. Tang J, et al. (1999) Proc Natl Acad Sci USA; 96(17): 9775-9780. Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774. Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844.

• All Sites:

  Human: Tyr319; Mouse: Tyr318; Rat: Tyr314

Western blot analysis of extracts from Jurkat cells,using Zap-70 (Ab-319) antibody (Line1 and 2) and Zap-70 (Phospho-Tyr319) antibody (Line 3 and 4).