• Catalog Number:

  E1A8254B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human ITCH around the phosphorylation site of tyrosine 420 (F-I-YP-G-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q96J02

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  AIF4; AIP4; atrophin-1-interacting protein 4; EC 6.3.2.-; Itchy homolog E3 ubiquitin protein ligase; NAPP1; NFE2-associated polypeptide 1; ubiquitin protein ligase ITCH

• Specificity Sensitivity:

  ITCH (Ab-420) antibody detects endogenous levels of total ITCH protein.

• References:

  Chen X., Genomics 73:238-241(2001). Deloukas P., Nature 414:865-871(2001). Wood J.D., Mol. Cell. Neurosci. 11:149-160(1998). ------------------------------------------------------------------------------------------------------------------------------------------

Western blot analysis of extracts from mouse brain cells, using ITCH (Ab-420) antibody.