• Catalog Number:

  E012066-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CHK1 around the phosphorylation site of serine 317 (S-S-SP-Q-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Rat

• SwissProtNo:

  O14757

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  CHK1 (Phospho-Ser317) antibody detects endogenous levels of CHK1 only when phosphorylated at serine 317.

• References:

  Dephoure N, et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7. Cruet-Hennequart S, et al. (2008) DNA Repair (Amst) 7, 582-96. Matsuoka S, et al. (2007) Science 316, 1160-6. Carlessi L, et al. (2007) Mol Cancer Ther 6, 935-44. Stiff T, et al. (2006) EMBO J 25, 5775-82.

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using CHK1 (Phospho-Ser317) Antibody (E012066).