• Catalog Number:

  E1A8139B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human MAP2K3 around the phosphorylation site of threonine 222 (A-K-TP-M-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P46734

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  dual specificity mitogen-activated protein kinase kinase 3; EC 2.7.12.2; kinase MKK3; MAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MEK3; MP2K3; PRKMK3

• Specificity Sensitivity:

  MAP2K3 (Ab-222) antibody detects endogenous levels of total MAP2K3 protein.

• References:

  Derijard B.,Science 267:682-685(1995). The MGC Project Team;Genome Res. 14:2121-2127(2004). Lim S.,J. Biol. Chem. 277:25040-25046(2002). ------------------------------------------------------------------------------------------------------------------------------------------

Western blot analysis of extracts from Jurkat cells, treated with serum (20%, 15mins), using MAP2K3 (Ab-222) antibody.