Rabbit IgG in phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human SEK1/MKK4 around the phosphorylation site of Threonine 261.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P45985
Applications:
IHC: 1:50~1:100 ELISA: 1:20000
Specificity Sensitivity:
SEK1/MKK4 (Ab-261) Antibody detects endogenous levels of total SEK1/MKK4 protein.
References:
Wang W, et al. (2002) Mol Cell Biol; 22(10): 3389-403.
Leung IW, et al. (2001)J Biol Chem; 276(3): 1961-7.
Immunohistochemical analysis of paraffin-embedded breast carcinoma, using SEK1/MKK4 (Ab-261) Antibody (#B7216). Left: Untreated; Right: Treated with synthesized peptide.