• Catalog Number:

  E011182-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ADD1 around the phosphorylation site of serine 726 (T-P-S P-F-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35611

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  ADD1 (Phospho-Ser726) antibody detects endogenous levels of ADD1 only when phosphorylated at serine 726.

• References:

  Pariser H, et al. Proc Natl Acad Sci USA 2005 Aug 30; 102(35): 12407-12 Tamaru S, et al. Biochem Biophys Res Commun 2005 Jul 01; 332(2): 347-51 Barkalow KL, et al. J Cell Biol 2003 May 12; 161(3): 557-70

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using ADD1 (Phospho- Ser726) antibody (E011182).

Western blot analysis of extract from HT-29 cells untreated or treated with Doxorubicin (1mM, 30min), using ADD1 (Ab-726) antibody (E021189, Lane 1 and 2) and ADD1 (Phospho- Ser726) antibody (E011182, Lane 3 and 4).