• Catalog Number:

  E1A7173B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  This antiserum was produced against synthesized non-phosphopeptide derived from human NF-κB p65 around the phosphorylation site of threonine 435.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q04206

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  NF-κB p65 (Ab-435) antibody detects endogenous levels of total NF-κB p65 protein.

• References:

  Yeh PY, et al. (2004) J Biol Chem.

Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NF-κB p65 (Ab-435) antibody (#B7173).

Western blot analysis of extracts from COS7 cells using NF-κB p65 (Ab-435) antibody (#B7173, Line 1 and 2) and NF-κB p65 (phospho-Thr435) antibody (#11012, Line 3 and 4).