Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human MSK1 around the phosphorylation site of serine 376 (G-Y-SP-F-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
O75582
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000
Other Names:
90 kDa ribosomal protein S6 kinase, , polypeptide 5; EC 2.7.11.1; kinase MSK1; KS6A5; MSPK1; Nuclear mitogen- and stress-activated protein kinase-1; ribosomal protein S6 kinase, polypeptide V; RLPK; RPS6KA5
Specificity Sensitivity:
MSK1 (Ab-376) antibody detects endogenous levels of total MSK1 protein.
References:
Deak M., EMBO J. 17:4426-4441(1998).
New L., J. Biol. Chem. 274:1026-1032(1999).
Jiang C., Cytogenet. Cell Genet. 87:261-262(1999).
Peptide - +
Western blot analysis of extracts from HT-29 cells, using MSK1 (Ab-376) antibody.
Immunofluorescence analysis of A549 cells, using MSK1 (Ab-376) antibody.