Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human c-Kit around the phosphorylation site of tyrosine 721 (N-E-YP-M-D).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
c-Kit (Ab-721) antibody detects endogenous levels of total c-Kit protein.
References:
G. Sakuntala Warshamana-Greene Mol, et al. (2004) Cancer Ther. 3: 527-536
Y Kubota, et al. (1998) Cell Growth Differ. 9: 247-256
Pramod Pandey, et al. (1995) Cancer Res. 55: 4000-4003.
Nancy K. Pryer, Clin. et al. (2003) Cancer Res. 9: 5729-5734
Western blot analysis of extracts from MO7e cells untreated or treated with SCF (50 ng/ml for 2 min), using c-Kit (Ab-721) antibody (Line 1 and 2) and c-Kit (Phospho-Tyr721) antibody (Line 3 and 4).