Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Lck around the phosphorylation site of tyrosine 393 (N-E-YP-T-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P06239
Applications:
WB: 1:500~1:1000 ELISA: 1:40000
Alter Name:
LSK, LSK-T, P56-LCK, T cell-specific protein-tyrosine kinase, kinase Lck
Specificity Sensitivity:
Lck (Ab-393) antibody detects endogenous levels of total Lck protein.
References:
Lara K. Yurchak, J. Biol. Chem., May 1996; 271: 12549.
Yuhong Liu, J. Biol. Chem., Feb 2000; 275: 3603.
LA Lewis, J. Immunol., Sep 1997; 159: 2292.
U Lorenz, Mol. Cell. Biol., Mar 1994; 14: 1824 - 1834.
Western blot analysis of extracts from 293 cells treated with serum (15%, 10min), using Lck (Ab-393) antibody (#B7139, Line 1 and 2).