• Catalog Number:

  E1A8230A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BL-CAM around the phosphorylation site of tyrosine 807 (G-D-YP-E-N).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P20273

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Leu-14; Siglec-2

• Specificity Sensitivity:

  BL-CAM (Phospho-Tyr807) antibody detects endogenous levels of BL-CAM only when phosphorylated at tyrosine 807.

• References:

  Wilson G.L., J. Exp. Med. 173-137-146(1991). Wilson G.L., J. Immunol. 150:5013-5024(1993). Stamenkovic I., Nature 345-74-77(1990).

• All Sites:

  Human: Tyr807

Western blot analysis of extracts from K562 cells, treated with Na3VO4 (0.3nM, 40mins), using BL-CAM (Phospho-Tyr807) antibody.