• Catalog Number:

  E1A7124B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human IκB-α around the phosphorylation site of Serine 32/36.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P25963

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Specificity Sensitivity:

  IκB-α (Ab-32/36) Antibody detects endogenous levels of total IκB-α protein.

• References:

  Mattioli I, et al. (2004)J Immunol; 172(10): 6336-44. Courtois G, et al. (2003)J Clin Invest; 112(7): 1108-15. Nair A, et al. (2003) Oncogene; 22(1): 50-8. Fan C, et al. (2002)J Cell Sci; 115(Pt 24): 4843-53. Schubert SY, et al. (2002)FASEB J; 16(14): 1931-3.

Immunohistochemical analysis of paraffin-embedded breast carcinoma, using IκB-α (Ab-32/36) Antibody (#B7124). Left: Untreated; Right: Treated with synthesized peptide.

Western blot analysis of extracts from MCF7 cells treated with TNF-α: Line1: Using IκB-α (Ab-32/36) Antibody (#B7124); Line2: The same antibody pre- incubated with synthesized peptide.