• Catalog Number:

  E1A8286A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AIRE around the phosphorylation site of serine 156 (P-G-SP-Q-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  O43918

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  AIRE1; APECED; APS1; APSI; autoimmune polyendocrinopathy candidiasis ectodermal dystrophy; PGA1

• Specificity Sensitivity:

  AIRE (Phospho-Ser156) antibody detects endogenous levels of AIRE only when phosphorylated at serine 156.

• References:

  Nagamine K., Nat. Genet. 17:393-398(1997). Aaltonen J., Nat. Genet. 17:399-403(1997). Hattori M., Nature 405:311-319(2000).

• All Sites:

  Human: Ser156

Western blot analysis of extracts from HeLa cells, treated with Hu (2nM, 24hours), using AIRE (Phospho-Ser156) antibody.