Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 639 (P-K-SP-V-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P35568
Applications:
IHC: 1:50~1:100 ELISA: 1:20000
Alter Name:
IRS-1, IRS1, Insulin receptor substrate-1
Specificity Sensitivity:
IRS-1 (Ab-639) antibody detects endogenous levels of total IRS-1 protein.
References:
Gao, Z. et al. (2002) J. Biol. Chem. 277, 48115-48121.
Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447.
Ozes, O.N. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 4640-4645.
Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1 (Ab-639) antibody (#B7123).