• Catalog Number:

  E1A7104B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human HER2 around the phosphorylation site of Tyrosine1248.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P04626

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Specificity Sensitivity:

  HER2 (Ab-1248) Antibody detects endogenous levels of total HER2 protein.

• References:

  Ramsauer VP, et al. (2003)J Biol Chem; 278(32): 30142-7. Jepson S, et al. (2002)Oncogene; 21(49): 7524-32. Ren Z, et al. (2002)J Biol Chem; 277(41): 38486-93.

Eppenberger-Castori S, et al. (2001)J Clin Oncol; 19(3): 645-56.

Immunohistochemical analysis of paraffin- embedded breast carcinoma, using HER2 (Ab-1248) Antibody. Left: Untreated; Right: Treated with synthesized peptide.

Western blot analysis using HER2(Ab-1248) Antibody and HER2(Phospho-Tyr1248) Antibody.Line1: The extracts from A2780 cells; Line2: The extracts from HeLa cells; Line3: The extracts from SK-OV3 cells; Line4: The extracts from SK-OV3 cells preincubated with synthesized peptide.