Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human c-Jun around the phosphorylation site of Serine 63.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P05412
Applications:
WB: 1:500~1:1000 ELISA: 1:5000
Specificity Sensitivity:
c-Jun ( Ab-63) Antibody detects endogenous levels of c-Jun protein around Serine 63.
References:
Wei W, et al. (2005). Cancer Cell. 8(1): 25-33.
Sevilla A, et al. (2004). Oncogene.23(55): 8950-8958.
Li L, et al. (2004). J Biol Chem.279(6): 4058-4065.
Hurd C, et al. (2002). Oncogene.21(14): 2154-2160.
Western blot analysis of extracts from NIH/3T3 cells treated with UV. Line1: Using c-Jun(Ab-63) Antibody (#B7045); Line2:The same antibody preincubated with synthesized peptide.