Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human GIRK1/KIR3.1/KCNJ3 around the phosphorylation site of serine 185 (K-M-SP-Q-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P48549
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000
Other Names:
G protein-activated inward rectifier potassium channel 1, IRK3, Inward rectifier K channel Kir3.1, KCNJ3, Kir3.1, Potassium channel, inwardly rectifying, subfamily J, member 3
Specificity Sensitivity:
GIRK1/KIR3.1/KCNJ3 (Ab-185) antibody detects endogenous levels of total GIRK1/KIR3.1/KCNJ3 protein.
References:
Chan K.W., J. Gen. Physiol. 107:381-397(1996).
Schoots O., Brain Res. Mol. Brain Res. 39:23-30(1996).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Peptide - +
Western blot analysis of extracts from NIH/3T3 cells, using GIRK1/KIR3.1/KCNJ3 (Ab-185) antibody.
Immunofluorescence analysis of HeLa cells, using GIRK1/KIR3.1/KCNJ3 (Ab-185) antibody.