• Catalog Number:

  E1A0063B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human CDC25B around the phosphorylation site of serine 323 (S-P-S-M-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P30305

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

• Alter Name:

  CDC25HU2, CDC25M2, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M-phase inducer phosphatase 2, MPIP2

• Specificity Sensitivity:

  CDC25B (Ab-323) antibody detects endogenous levels of total CDC25B protein.

• References:

  Stéphanie Dutertre, J. Cell Sci., May 2004; 117: 2523 - 2531. Véronique Baldin, J. Biol. Chem., Dec 1997; 272: 32731. Estelle Schmitt, J. Cell Sci., Oct 2006; 119: 4269 - 4275.

Peptide - + Western blot analysis of extracts from RAW264.7 cells, treated with Hu (2nM, 24hours), using CDC25B (Ab-323) antibody (#B0063, Line 1 and 2). Immunofluorescence analysis of HuvEc cells, using CDC25B (Ab-323) antibody (#B0063).