• Catalog Number:

  E1A0798A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p38 MAPK around the phosphorylation site of tyrosine 322 (D-P-YP-D-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q16539

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  CRK1, CSAID binding protein, CSBP, CSBP1, CSBP2, Cytokine suppressive anti- inflammatory drug binding protein, EC 2.7.11.24, MAP kinase MXI2, MAP kinase p38, MAPK14, MAX-interacting protein 2, MK14, MXI2, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38, kinase p38-alpha

• Specificity Sensitivity:

  p38 MAPK (Phospho-Tyr322) antibody detects endogenous levels of p38 MAPK only when phosphorylated at tyrosine 322.

• References:

  Nasreen Khalil, J. Biol. Chem., Dec 2005; 280: 43000 - 43009. Monica Ambrose, AACR Meeting Abstracts, Apr 2005; 2005: 241. Hiroe Shiratsuchi, Am J Physiol Renal Physiol, Oct 2002; 283: 678. Rocky Pramanik,J. Biol. Chem., Feb 2003; 278: 4831 - 4839.

• All Sites:

  Human: Tyr322; Mouse: Tyr322; Rat: Tyr322

Western blot analysis of extracts from Jurkat cells, using p38 MAPK (Phospho-Tyr322) antibody.