Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human MMP-2.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P08253
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Alter Name:
CLG4A, COLLAGENASE TYPE IV, 72-KD, COLLAGENASE TYPE IV-A,GELATINASE, 72-KD, GELATINASE, NEUTROPHIL, matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Specificity Sensitivity:
MMP-2 antibody detects endogenous levels of total MMP-2 protein.
References:
Charlotte J. Morrison, J. Biol. Chem., Dec 2001; 276: 47402 - 47410.
Hala S. Azzam, J Natl Cancer Inst, Nov 1993; 85: 1758 - 1764.
Jane L. English, J. Biol. Chem., Apr 2006; 281: 10337 - 10346.
JORDI ESPARZA, FASEB J, Nov 2004; 18: 1682 - 1691.
Western blot analysis of extracts from LOVO cells, using MMP-2 antibody (#C0270).