Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PLC-γ1 around the phosphorylation site of tyrosine 771 (P-D-YP-G-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P19174
Applications:
WB: 1:500~1:1000 IHC:1:50~1:100
Specificity Sensitivity:
PLC-γ1 (Ab-771) Antibody detects endogenous levels of total PLC-γ1 protein.
References:
Singer, W. D. et al. (1997) Annu. Rev. Biochem. 66, 475-509.
Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027.
Wang, Z. et al. (1998) Mol. Cell. Biol. 18, 590-597.
Kim, H. K. et al. (1991) Cell 65, 435-441.
Margolis, B. et al. (1989) Cell 57, 1101-1107.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PLC-γ1 (Ab-771) Antibody (E021523).
Western blot analysis of extracts from 293 cell using PLC-γ1 (Ab-771) Antibody (E021523, Lane 1 and 2 )