• Catalog Number:

  E1A0167L-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from human CASP8.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  Q14790

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  EC 3.4.22.61, CASP-8, ICE-like apoptotic protease 5, MORT1-associated CED-3 homolog, MACH, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, FLICE, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, Caspase-8 subunit p18, Caspase-8 subunit p10, CASP8, MCH5, Caspase-8 [Precursor]

• Specificity Sensitivity:

  CASP8 (Cleaved-Asp384) antibody detects endogenous levels of fragment of activated CASP8 resulting from cleavage adjacent to Asp384.

• References:

  Boldin M.P., Cell 85:803-815(1996). Muzio M., Cell 85:817-827(1996). Fernandes-Alnemri T., Proc. Natl. Acad. Sci. U.S.A. 93:7464-7469(1996).

Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 1hour), using CASP8 (Cleaved-Asp384) antibody.