Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human CD226/DNAM-1 around the phosphorylation site of serine 329 (T-F-SP-R-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
Q15762
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Other Names:
CD226, DNAM1, DNAX accessory molecule-1, PTA1, TLiSA1, adhesion glycoprotein, platelet and T cell activation antigen 1
Specificity Sensitivity:
CD226/DNAM-1 (Ab-329) antibody detects endogenous levels of total CD226/DNAM-1 protein.
References:
Shibuya A., Immunity 4:573-581(1996).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Tahara-Hanaoka S., Int. Immunol. 16:533-538(2004).
Peptide - +
Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using CD226/DNAM-1 (Ab-329) antibody.
Immunofluorescence analysis of HeLa cells, using CD226/DNAM-1 (Ab-329) antibody.