Western blot analysis of extracts of various cell lines, using FBP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunofluorescence analysis of MCF-7 cells using FBP1 antibody. Blue: DAPI for nuclear staining.