Western blot analysis of extracts of HepG2 cells, using DSP antibody at 1:800 dilution. Secondary antibody: HRP Goat AntiRabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.(A7635) at 1:1000 dilution. Secondary antibody: HRP Goat Anti Rabbit IgG(H+L) at 1:10000dilution. Lysates /proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
