Western blot analysis of extracts of Raji cells, using PTDSS1 antibody(E913065) at 1:3000 dilution. Secondary antibody: HRP Goat Anti Rabbit IgG (H+L) at 1:10000dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.