Western blot - SLC47A1 Polyclonal Antibody.Western blot analysis of extracts of rat liver, using SLC47A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 30s.