Western blot analysis of extracts of various cell lines, using DSP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using DSP antibody at dilution of 1:100 (40x lens).
