WB analysis of Hela lysis using S100A1 antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000.
Immunohistochemical analysis of paraffin- embedded human-brain, antibody was diluted at 1:200.
Immunohistochemical analysis of paraffin-embedded Human kidney. Antibody was diluted at 1:200(4°,overnight). High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. Secondary antibody was diluted at 1:200(room temperature, 30min).