WB analysis of 293T, 3T3 lysis using DPP4 antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000.
Immunohistochemical analysis of paraffin-embedded Human kidney. Antibody was diluted at 1:100(4℃,overnight). High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. Secondary antibody was diluted at 1:200 (room temperature, 30min).
