Immunohistochemical analysis of paraffin- embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris- EDTA, pH8.0 was used for antigen retrieval. Negative control (right) obtained from antibody was pre-absorbed by immunogen peptide.
WB analysis of lysates from Jurkat cells treated with insulin 0.01U/ml 15', using PER3 Antibody. The lane on the right is blocked with the synthesized peptide.
