Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°, overnight). High- pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negative control (right) obtained from antibody was pre-absorbed by immunogen peptide.
WB analysis of lysates from Jurkat, HepG2, and A549 cells, using EIF5B Antibody. The lane on the right is blocked with the synthesized peptide.
