Immunohistochemical analysis of paraffin- embedded Human Amygdala. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
WB analysis of lysates from Raw264.7 cells treated with UV 15', using CDC25B Antibody. The lane on the right is blocked with the synthesized peptide.
